Fig. 1. Different migratory cell types behaved normally in ß1D1D ki/ki embryos. (A-D) Transverse sections showing (A,D) snail expression in migratory neural crest cells (arrows) and (B,E) pax3 expression in migratory limb muscle precursor cells (arrows) of E9.5 heterozygous (A,B) and ß1D1D ki/ki (D,E embryos). (C,F) PGC distribution was analysed in transverse serial sections of E10.5 wild-type (C) and ß1D1D ki/ki (F) embryos. An enlargement of the right gonadal ridge is shown. (G,H) At E10.5, PGCs were detected by AP staining in the hindgut (hg), mesenterium (m), gonadal ridges (gr) and ectopic regions (o) of ß1D1D ki/ki, heterozygous and wild-type embryos in the quantities indicated. (I) RT-PCR detection of ß1A and ß1D in isolated PGCs from different stages. Oct4 is a PGC marker at these stages. HPRT is a loading control. ba, first branchial arch; fb, forebrain; gr, gonadal ridges; h, newborn heart; l, limb bud; nt, neural tube; s, E11.5 gonadal somatic tissue. Scale bars: 200 µm (A,B,D,E), 400 µm (C,F).