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Fig. 1. Deletion of PTEN in PGCs. Immunohistochemical staining for PTEN was carried out on E15.5 control (A, Ptenflox/–: TNAP/Cre–) and mutant (B, Ptenflox/–: TNAP/Cre+) testes. In control mice, strong cytoplasmic staining was observed in germ cells but the expression level was low in somatic cells. In mutant mice, only background level of expression was observed both in germ and somatic cells. Scale bar: 20 µm.