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Fig. 3. Apoptosis in Pten mutant mice. (A) Sections of embryo testes from E13.5, E14.5 and E15.5 control and mutant mice were analyzed by the TUNEL assay. Scale bars: 20 µm. (B) The percentages of apoptotic PGCs at E13.5, E14.5 and E16.5. The percentage of apoptotic cells in Mvh-positive germ lineage cells (mean±s.d.) was calculated and analyzed using Student's t-test (P=0.22 at E13.5, *P<0.01 at E14.5 and {dagger}P<0.001 at E16.5). Three to six mice were examined at each stage.