Fig. 7. Differentiation capacity of Pten mutant-derived EG cells. (A) Embryoid bodies with various cell types formed when differentiation was induced in methylcellulose cultures for 8 days. Scale bars: 50 µm. (B) RNA from embryoid bodies was subjected to semi-quantitative RT-PCR analysis to examine the expression of ectodermal (Wnt1), endodermal (collagen IV) and mesodermal (T) markers. ß-Actin was used as a loading control. Expression of all three markers was induced both in control and in mutant EG cell-derived embryoid bodies. (C) Teratomas with differentiated cells that formed when EG cells were transplanted into nude mice. Scale bars:50 µm.