Fig. 3. Analysis of markers at day 15 of pregnancy. (A) RTD-PCR was used to compare expression of HIF1 target genes following normalization to Ck19 (mean±s.e.m.). (B) The mRNA expression levels of several known markers of mammary epithelial cell differentiation, including ß-casein, -lactalbumin (-lac), Xor, Adfp and perilipin were analyzed by semi-quantitative RT-PCR. The graph indicates the average level of expression per gene normalized to ß-actin (±s.e.m.).