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Fig. 6. Localization of Inv::GFP to 9+0 cilia but not to 9+2 cilia. Sections of the kidney (A-C), pituitary (D-F), retina (G,H), cerebellum (I), oviduct (J-L) and trachea (M-R) of Inv/Inv, Inv::GFP mice were subjected to double immunostaining with antibodies to GFP (red) (A,D,G,I,J,M) and antibodies to acetylated tubulin (green) (B,E,K,N) or with antibodies to GFP (red; P) and antibodies to {gamma}-tubulin (green; Q). DIC images are shown at the right of corresponding fluorescence images (C,F,H,L,O,R). Note the prominent localization of Inv::GFP in 9+0 cilia (arrowheads) of kidney (A-C), pituitary gland (D-F) and retinal (G,H) cells. The fusion protein was not detected in the 9+2 cilia (arrowheads) of oviduct (J-L) or tracheal (M-O) cells, but was detected in the cytoplasm (arrow) of the Purkinje cells (I, arrow) and in the basal body (arrows) of tracheal cells (P-R, arrows). Both 9+0 and 9+2 cilia were positive for acetylated tubulin (B,E,K,N) and the basal body was positive for {gamma}-tubulin (Q). Pt, proximal tubule; Rpe, retinal pigment epithelium.