Fig. 7. Localization of calmodulin to 9+2 cilia. Sections both of the trachea (A-C) and kidney (D-F) of newborns and of the node (G-I) of embryos at E8.0 were subjected to double staining with antibodies to acetylated tubulin (green) (A,D,G) and antibodies to calmodulin (red) (B,E,H). Corresponding DIC images are shown on the right of each pair of fluorescence images (C,F,I). Calmodulin was localized to the 9+2 cilia (arrowheads) of tracheal cells, but not in the 9+0 cilia (arrowheads) of the kidney and node.