Fig. 8. Expression of PAX6 and Foxe3 in Mab21l1^+/- and Mab21l1^-/- embryos. In situ hybridization (A,B,E,F,I,J) or immunohistochemistry (C,D,G,H) of Mab21l1^+/- and Mab21l1^-/- frozen sections at 28- (A-D) and 35- (E-H) somite stages, and paraffin wax sections at the 40-somite stage (I,J). Expression of: Foxe3 transcripts in (A,E,I) Mab21l1^+/- and (B,F,J) Mab21l1^-/- embryos; (C,G) PAX6 protein in Mab21l1^+/- and (D,H) Mab21l1^-/- embryos. Expression of Foxe3 is reduced in Mab21l1^-/- presumptive lens placode (B, arrowheads), whereas expression of PAX6 is maintained at the 28-somite stage (D). At the 35-somite stage, Foxe3 is expressed in some cells (F, arrowhead), whereas PAX6 is expressed in most cells in the Mab21l1^-/- lens lineage structure (H). By the 40-somite stage, Foxe3 expression has disappeared (J). Scale bars: 50 µm.