Fig. 3. The KG02598 P-element insertion reduces CG8667 expression and is a strong dimm mutant allele. (A) Photomontages of CG8667 in situ hybridization in homozygous stage 16 and 17 embryonic CNS: normal staining levels were observed in 7/15 specimens from the stock dimm^KG02598/CyO-y⁺ (left); reduced staining levels were observed in the remaining eight specimens (right). In the presumed homozygotes, residual staining was seen only in unpaired cell bodies in caudal abdominal neuromeres that normally stain strongly (arrow). Normal staining levels were also found in 15 out of 15 comparable specimens from the cross dimm^KG02598/CyO-y⁺ x Canton S (not shown). (B) Reduction in immunostaining with the PT2 antiserum in the CNS of a first instar dimm^KG02598/Rev4 larva (B'), compared with a dimm^+/- sibling control (B) and a dimm^S2a/Rev4 control (B''). dimm^S2a is a precise excision of the KG02598 P-element. (C). Photomontages of reduced Fmrf in situ hybridization in the CNS of a first instar dimm^KG02598/Rev4 larva (C') compared with a dimm^+/- sibling control (C). Scale bars: 50 µm.