(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 7. dimm controls levels of ectopic neuropeptide (proPDF) but not levels of ectopic pro-pdf mRNA. (A) Brightfield photomontages of pdf mRNA in situ hybridization in the dorsal chain neurons of dimm+/- (A; R6, ap-Gal4/+; UAS-pdf/+) and dimm-/- (A'; R6, ap-Gal4/Rev8; UAS-pdf/+) third instar stage CNS. Heterologous expression of the pdf neuropeptide gene, which encodes the PAP neuropeptide, was directed to these neurons using UAS-pdf (Renn et al., 1999) and ap-GAL4. (B) Mean pixel intensity (intensity index) for pdf mRNA in situ hybridization in the somata of selected pairs of c929-positive dorsal neurons (dimm+/-, n=11-12; dimm-/-, n=8-9). (C) Confocal z-series images of dorsal PAP (proPDF) immunostaining in the CNS from dimm-/+ (C) and dimm-/- (C') third instar larvae. Reduced staining (C') was observed in the dorsal chain neurons (d1-11) and the dorsal neurohemal organs (asterisks), which contain the neuroendocrine terminals of the Tv neuron (Benveniste and Taghert, 1999). Note that staining levels in c929-negative cells were unaffected: these included PN (natively pdf-positive), and v7 and v8 (ectopic, ap-Gal4-dependent expression). (D) Mean pixel intensity (intensity index) for PAP immunostaining in the somata of selected pairs of dorsal neurons and in the PN neurons (dimm+/-, n=6; dimm-/-, n=5). (E) Confocal z-series images of ventral PAP immunostaining in T2v (arrows) and in the ventral chain neurons, including v2a and v8, of a dimm-/+ third instar stage CNS (E; same CNS as C) and a dimm-/- CNS (E'; same CNS as C'). The Tv neurons (arrows) express the c929 reporter gene (Fig. 1C,E) and display the dimm mutant phenotype. By contrast, the ventral chain neurons do not express the reporter gene (data not shown), and they do not display the dimm mutant phenotype. (F) Mean pixel intensity (intensity index) for PAP immunostaining in the somata of selected pairs of ventral neurons (dimm+/-, n=6; dimm-/-, n=5). The brightest cell in each Tv cluster was assumed to be Tvb (P.H.T. and M. Han, unpublished). *P<0.05, **P<0.01, ***P<0.001. Scale bars: 50 µm.