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Fig. 1. Isolation of a novel neuralizing factor. (A) Animal caps expressing ß-globin (a,c) and A124C10 (b,d) at the equivalent of stage 25. Whole-mount in situ analysis for nrp1 expression was carried out in c,d. Arrowheads, cement glands. (B) RT-PCR analysis for neural marker expression in ß-globin or A124C10 expressing animal caps at early gastrula (stage 10.5) and tailbud (stage 25) stages. RT(+) and RT(-) indicate whole-embryo RNA transcribed with or without reverse transcriptase, respectively. EF-1{alpha} was used as a loading control. Doses of injected mRNA (pg/embryo): ß-globin, 500; A124C10, 500.