Fig. 2. Structural features of XMAN1. (A) Schematic structural comparison of human (Accession Number, AF112299) and Xenopus MAN1 (Accession Number, AB100267). LEM domains (blue), putative transmembrane domains (TM, yellow), and RNA recognition motif (RRM, red) are indicated. RNP2 and RNP1, conserved regions among RRMs, are shown as black boxes. A black bar represents the coding region of A124C10. The total numbers of amino acid residues of each protein and sequence identities of each domain between the two proteins are shown. (B) The sequence of the LEM domain of Xenopus MAN1 in comparison with those of human MAN1, LAP2 and emerin. Asterisks and colons indicate identical and homologous amino acid residues, respectively, conserved among at least three molecules. (C) Sequence comparison between human and Xenopus MAN1 from the first transmembrane domain. Asterisks and colons are indicated as above. RRM domains are boxed. (D) Alignment of putative RRMs of human and Xenopus MAN1. Pfam00076 represents an RRM in a database for multiple sequence alignments (Bateman et al., 2002). A core sequence for RRM is reported previously (Birney et al., 1993). U, uncharged; Z, uncharged, serine or threonine; x, any amino acid residues.