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Fig. 3. Temporospatial expression of XMAN1. (A) Analysis of temporal expression pattern of XMAN1 by RT-PCR with RNA from various developmental stages. FGFR, a loading control. (B) Whole-mount in situ analysis of Xenopus embryos at early gastrula (a-c), neurula (d-f) and tadpole (g,h) stages. c and e show cross-sections of b and d, respectively. f represents a close view of the dorsal region of e. h shows a dorsal view of the head region of g. Arrows, dorsal blastopore groove. nc, neural crest; nt, notochord; fp, floor plate; el, epithelial layer; sl, sensory layer; ey, eye; ov, otic vesicle; ba, branchial arches.