Fig. 4. Neuralizing activity of XMAN1 resides in the C-terminal region. (A) Schematic representation of XMAN1 deletion mutants. LEM domains, transmembrane domains and RRMs are depicted as in Fig. 2A. Circles indicate Myc tags. (B) Western blot for analyzing the expression level of the XMAN1 deletion mutants. Extracts prepared from stage 11 gastrula embryos expressing XMAN1 deletion mutants were immunoblotted with anti-Myc antibody (upper panel) or anti-ß-tubulin antibody (lower panel; loading control). (C) Mapping of neuralizing activity of XMAN1. RT-PCR analysis for nrp1 was performed with animal caps at the equivalent of the tailbud stage 25. Doses of injected mRNA: 500 pg per embryo. (D) Confocal microscopic analysis of the subcellular localization of Myc-tagged XMAN1 (a-c), Flag-tagged XMAN1-CT (d-f) and Myc-tagged human emerin (g-i) expressed in COS-7 cells (green). Nuclei (red) were visualized by co-transfecting with the DsRed2-Nuc vector. Scale bars: 10 µm.