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Fig. 5. XMAN1 dorsalizes the ventral mesoderm. (A) Embryos were injected with ß-globin (a,c,e) or XMAN1-CT (b,d,f) mRNA into the ventral marginal zone at the four-cell stage and observed at stage 20 (a,b) or stage 35 (c-f). Somites were stained with 12/101 antibody (e,f). Arrowheads, secondary axes. (B) RT-PCR analysis of RNA from ß-globin or XMAN1-CT mRNA (500 pg per embryo)-injected ventral marginal zones for expression of pan-mesodermal (Xbra), dorsal (gsc, chordin and {alpha}-actin) and ventral (Xhox3, Msx1 and Xvent1) mesodermal markers.