Fig. 2. Comparison of Actin-GFP and Tau-lacZ profiles in mature embryonic peripheral glia. Embryos expressing UAS-actin-GFP and UAS-tau-lacZ using the repo:GAL4 driver were fixed and stained with anti-GFP and anti-ß-galactosidase, then viewed with laser scanning confocal microscopy. Anterior is towards the top, CNS is towards the left. Embryos are stage 16. (A) Actin-GFP channel (green). (B) Tau-lacZ channel (red). (C) Merge of A and B shows that within the cell, actin-GFP and tau-lacZ profiles show little direct overlap. However, at the leading edge of the growing vPG cell, both actin-GFP and tau-lacZ labeling is observed (arrows).