Fig. 8. Homeoprotein domains interacting with Foxa2, as revealed in pull-down assays. Summary of the protein domains within the four homeoproteins studied (other than Engrailed) that bind Foxa2 [Hoxa5, Gsc, Lim1 (this work) and Otx2 (Nakano et al., 2000)]. Radioactive fragments of the homeoproteins were tested for binding to Foxa2 (+ indicates positive binding; — indicates absence of binding). In all cases, the homeodomain (HD) binds Foxa2. Moreover, three homeoproteins out of four have, like Engrailed, one additional interacting domain, located either in their N-terminal domain (Hoxa5, Gsc) or in their C-terminal (Otx2) sequence. HP, Hexapeptide; GEH/Eh1, Gsc-Engrailed homology/Eh1 domain; LIM, LIM domains; Tail, tail domain of Otx2.