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Fig. 1. Levels of Fgf8 expression are reduced in Fgf8neo/neo embryos. (A-B') Whole-mount Fgf8 in situ hybridization on E9.5 wild-type (A,A') and Fgf8neo/neo (B,B') embryos. Lateral views (A,B) show that Fgf8 expression domains in the midbrain-hindbrain boundary (white arrows) and in the rostral midline (black arrowheads) are present but reduced in size and intensity in Fgf8neo/neo embryos. Frontal views of the same embryos (A',B') show changes in both the commissural plate (black arrowheads) and the olfactory placodes (black arrow), the two sites of Fgf8 expression in or in the vicinity of the telencephalic anlage. (C,D) Fgf8 in situ hybridization on horizontal sections of wild-type (C) and Fgf8neo/neo (D) E12 embryos. In Fgf8neo/neo embryos, the neocortex primordium and the bulks of the ventral telencephalic ganglionic eminences are present. The rostral midline is hypoplastic and the width of Fgf8 expression domain (black arrowheads) is reduced. LGE, lateral ganglionic eminence; MGE, medial ganglionic eminence; Ncx, neocortex; Tel, telencephalon.