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Fig. 7. Topographic organization of thalamocortical projections in Fgf8neo/neo neonates. DiI and DiA crystals or single DiI crystals were placed in the occipital (Occ) and parietal (Par) neocortex (A-B'), the caudal parietal neocortex (C-D'), the parietal neocortex (E-F') and the rostral parietal and frontal (Fr) neocortex (G-H'). In A-H, crystal placements (red and green arrowheads) are shown in lateral (A,B) or dorsal views (C-H) of the brain hemispheres. These placements are shown before dye diffusion, except in A,B. (A'-H') Dorsal thalamus coronal sections of the corresponding brains after dye diffusion. The retroflexus tract (white arrowhead) and the external medullary lamina (broken line) are morphological landmarks used to position and identify presumptive thalamic nuclei. Sections are counterstained with Hoechst. (A-B') DiI injection in the occipital neocortex and parietal neocortex retrogradely stain the same presumptive thalamic nuclei in Fgf8neo/neo brains. The dorsal lateral geniculate nucleus (dLGN) is labeled in pink/red with DiI and the lateral part of the ventroposterior complex (VP) is labeled in green with DiA. (C-D') Large DiI injections in the caudal parietal neocortex normally label cells in the lateral VP domain and spread into the presumptive lateral posterior nucleus (LP) (open arrowheads) in both wild type and mutant neonates. In this particular Fgf8neo/neo neonate, the injection also labeled a few cells located more lateroventrally in the dLGN (arrows). (E-F') Small injections of DiI and DiA into the parietal neocortex label two overlapping domains within the VP complex in both control and Fgf8neo/neo newborns. (G-H') Small injections of DiI into the orbitofrontal/frontal neocortex and of DiA in the rostral parietal neocortex label a medial VP domain as well as a more medial domain in both wild-type and Fgf8neo/neo newborns. White arrowheads in C',D',E',F',G',H' indicate the position of the fasciculus retroflexus. dLGN, dorsal lateral geniculate nucleus; LP, lateroposterior nucleus; M, midbrain; n/n, Fgf8neo/neo; Ncx, neocortex; OB, olfactory bulb; VP, ventroposterior nucleus.