Fig. 8. Zic1 represses Cath1 and Cash1 but not chick Ngn1 and Ngn2. (A-D) Neural tubes of HH14 chick embryos were electroporated on the right side (arrowheads) with ^mycZic1 and harvested at 24 hours. mRNA in situ hybridization with Cath1 (A), Cash1 (B), Ngn1 (C) and Ngn2 (D) are shown. Relative to the control side (left) in each case, Cath1 and Cash1, but not Ngn1/2, are repressed by overexpression of ^mycZic1. (E-H) Double label immunofluorescence using anti-Mash1 (red) and anti-Zic (green) antisera on E11.5 mouse spinal neural tube. Yellow cells indicate overlap in expression in the dorsal Mash1 domain (F-H, arrowheads). Scale bar: 100 µm in A-E; 50 µm in F-H.