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Fig. 2. Developmental northern blot of LvTbx2/3 expression. Poly(A)+ RNA (3 µg per lane) was loaded (calculated by OD260). Loading was verified by probing the blot with a ubiquitin fragment from L. pictus (data not shown). Egg; 60 cell stage; MB, mesenchyme blastula; EG, early gastrula; LG, late gastrula; PR, prism; PL, pluteus larva.