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Fig. 3. Tbx2/3 polyclonal and preimmune sera controls. Western-blot analysis of protein extracts from late gastrula (1500 embryos) using polyclonal LvTbx2/3 and preimmune serum. In blots probed with LvTbx2/3, two immunoreactive bands appear, one of ~70 kDa and one of 35 kDa. A 35 kDa band was also recognized by preimmune serum, indicating the presence of a nonspecific antigen.