Development -- Xu et al. 130 (9): 2001 Figure IG7

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Fig. 7. Absence of the Brca1 full-length isoform disrupted ${gamma}$ H2AX localization. Scp3 and ${gamma}$ H2AX double immunofluorescence localization of control and mutant spermatocytes. (A-D,F-I) Localization of ${gamma}$ H2AX in unirradiated testes. Localization is similar at the leptotene (Lep) and early zygotene (E. Zyg) stages in control and mutant spermatocytes. Starting from the late zygotene (L. Zyg) stage, ${gamma}$ H2AX localizes to the XY body in wild-type spermatocytes (arrows, C,D). However, in 90% of spermatocytes from Brca1 mutant mice, ${gamma}$ H2AX fails to localize to the XY body and remains as multiple foci at the late zygotene stage (H) and early pachytene (E. Pac) stage (I). (E,J) Localization of ${gamma}$ H2AX in early pachytene stage spermatocytes 10 minutes after 80 Gy irradiation. The XY body cannot be detected.