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Fig. 1. Early development of the OP lineage. (A) The OP proteloblast undergoes four asymmetric cell divisions to form the four primary op blast cells. The op4 blast cell is labeled red. The OP proteloblast undergoes a symmetric cell division to form the two O/P teloblasts. Each O/P teloblast then resumes asymmetric cell divisions to form the o and p bandlets. Blue arrows indicate the axis of cell division. (B) Divisions of the op4 blast cell. (C) Fluoromicrograph showing the four tertiary blast cells of the op4 clone (clonal age is 44 hours). The OP proteloblast was injected with rhodamine dextran immediately before the birth of the op4 blast cell. The specimen was counterstained with Hoechst 33258, and is shown with anterior toward the top. The boundary between the op4 clone and the o and p bandlets is marked by a yellow line. Note that the posterior edge of the op4.pp cell retains a unique chevron shape which borders the o bandlet to the future ventral side (left) and the p bandlet to the future dorsal side (right). Scale bar: 10 µm.