Fig. 7. Relationship between larval and adult ALs in early pupal stages. (A-C) Images of GAL4-GH146 driving membrane-targeted GFP. Each panel is a single confocal section through the developing AL stained with anti-GFP antibody (green), anti-N-cadherin antibody (red) and nc82 (blue). Dotted lines indicate the larval area of the lobe; solid lines indicate the adult-specific zone. Notice that, in C₄, nc82 is extremely weak in spite of an increase in laser power from 3% to 10% (A₄ and B₄, respectively). (D-F) z projections of confocal images of GAL4-MJ94 driving mCD8-GFP in larval ORNs. Asterisks indicate the ORN axons of the antennal nerve entering the larval AL; the arrow in F indicates the `stump' of remaining ORN axon terminals and the arrowheads indicate examples of nc82-positive densities that seem to lie along the few remaining ORN processes. Dotted lines indicate the extent of the larval AL. Green is mCD8-GFP; (D,F) magenta is nc82; (E) red is N-Cadherin and blue is nc82.