Fig. 8. Candidate cellular components of the developing adult AL. (A₁-A₅) Time course of repo-GAL4 driving mCD8-GFP expression, marking glial cell membrane. Each panel is a single optical section through the developing AL. (B₁-B₃) Comparison of repo-GAL4-driven marker expression, and anti-Repo antibody. Repo-GAL4 strongly labels all glia but has weak background staining in all cells. (B₄) Location of Repo-positive nuclei in relation to GH146-positive dendrites. (B₅) Triple stain of Repo, Elav and TOTO-3 (a DNA stain, most prominent in nucleoli) showing that Repo- and Elav-stained cells (glia and neurons, respectively) account for all nuclei in the vicinity of the AL. (C₁-C₅) Time course of GAL4-C155 driven UAS:mCD8-GFP (single sections); C₃ and C₅ correspond to the green channel of C₂ and C₄, respectively.