Fig. 6. Histology of ovaries obtained from control and rescued females at various ages. (A,C,E) Ovaries from control females (Brca2 Ko/+; Tg/+); (B,D,F) ovaries from rescued females (Brca2 Ko/Ko; Tg/+). (A,B) Ovaries from postnatal day 2 females stained with Weigert's Hematoxylin-picric acid Methyl Blue; (C,D) Three-week-old females; (E,F) Six-week-old females. The 3- and 6-week-old ovary sections are stained with Hematoxylin and Eosin. A marked reduction in the follicles was seen in the 6-week-old rescued ovaries. CL, corpus lutea; P, primordial follicles; Pr, primary follicles; Pa, preantral follicles; A, antral follicles; O, oocyte. Scale bars: 50 µm. (G) Table shows the number of follicles found in 3-week-old control and rescued female ovaries. The number of primordial and primary follicles were significantly reduced in rescued females.