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Fig. 3. Genes expressed in endoderm. (A) Venn diagram illustrating genes that were at least threefold enriched in unspecified endoderm as compared to adjacent mesoderm and ectoderm. 203 genes were enriched in the endoderm, whereas 262 genes were enriched in the mesectoderm. (B) The endodermal expression of four genes, Apo AIV, Dkk1, Prss12 and Eya2, identified from our microarray analysis, was verified by RT-PCR (end, endoderm; mec, mesoderm + ectoderm; HPRT is used as a control for RT-PCR) and whole mount ISH. These genes were not previously shown to be expressed in endoderm. The endodermal expression of Eya2 was verified by sectioning the stained embryo. ect, ectoderm; end, endoderm; mes, mesoderm.