Fig. 4. A summary of genes expressed in the pancreatic Pdx1-eGFP⁺ cells and non-pancreatic cells at E10.5 (including Pdx1-eGFP+ cells of the stomach + duodenum as well as PDX1-GFP^– cells). (A) Venn diagram showing that 158 genes (green) were enriched in the eGFP⁺ cells within the pancreatic region whereas 208 (red) genes were enriched in the PDX1⁺ cells in the duodenum and stomach or eGFP-cells. (B-E) Expression analyses of four genes by RT-PCR and in situ hybridization. ß actin expression was used as a control. (B) Spintz1(Af010499) was highly expressed in the pancreas and duodenum but not the stomach. (C) Tm4sf3 (AA571115) was expressed at a high level in the stomach and duodenum but not the pancreas. (D) Nkx2.2 was expressed at a higher level in the pancreas. (E) Osteopointin was only expressed in the pancreas. Black dashed lines, stomach; green dashed lines, duodenum; red dashed lines, pancreas.