Fig. 4. Lac co-localizes with SJs proteins. (A,B) Stage 14 wild-type embryo labelled with an antibody against Lac; two different focal planes are shown, trachea and epithelium (arrow and arrowhead, respectively, in A) and the CNS (arrow in B). (C-F) Details of the tracheal dorsal trunk (C,E) or the hindgut (D,F) of LacGFP stage 16 embryos labelled with anti-GFP to detect Lac accumulation (green), and an anti-Crb (C,D; red) or an anti-Arm antibody (E,F; red). (G,H) Detail of a stage 16 tracheal dorsal trunk (G-G₂) or the hindgut (H-H₂) of wild-type embryos, labelled with anti-Lac (G₁,H₁; green) and anti-Cora (G₂,H₂; red). Note, co-localization of the two proteins. (I,J) Detail of a stage 16 tracheal dorsal trunk (I-I₂) or the hindgut (J-J₂) of wild-type embryos labelled with an anti-Lac antibody (I₁,J₁; green) and a Nrx antibody (I₂,J₂; red).