Fig. 6. Lac is required for the distribution of SJs proteins, which are also required for tracheal morphogenesis. (A,B) Detail of the posterior part of stage 16 embryos, in dorsal view, 40 minutes to 1 hour after injecting dye. In the wild type (A), the tracheae (visible with transmission) remains non-permeable, whereas in Lac¹ mutants (B) the tracheae completely fills with dye. (C,D) Salivary glands of wild type (C) and Lac² mutants (D) labelled with anti-Cora antibody. (E,F) Detail of the dorsal trunk of wild type (E) or Lac² mutants (F) labelled with anti-Cora antibody. (G,H) Salivary glands of wild type (G) and Lac² mutants (H) labelled with anti-Dlg antibody. (I,J) Salivary glands of wild type (I) and cora⁵ mutants (J) labelled with anti-Lac antibody. (K,L) Detail of the dorsal trunk of wild type (K) or cora⁵ mutants (L) labelled with anti-Lac antibody. (M-N) Detail of 3-4 tracheal metameres of cora⁵ (M) or Nrx⁴³⁰⁴ (N) mutants stained with mAb2A12. Compare with Fig. 2A-D.