Fig. 5. Double in situ hybridization with probes against Hyzic, Cnash and Nowa, in all possible combinations. (A) Hyzic (red) and Cnash (blue) show no overlap in their expression patterns. (B) Similarly, Hyzic (red) and Nowa (blue) are expressed in different subpopulations of nematoblasts. (C) Only the messages of Cnash (blue) and Nowa (red) show a substantial co-localization, as demonstrated by the violet cell nests. (D) Quantitative analysis of the overlapping expression of Cnash and Nowa. Scale bars: 50 µm (A-C).