Fig. 5. Expression of CHL2 mRNA in soft tissues. (A) Northern blot analyses with human multiple tissue blots IV (lanes 1-8) and I (lanes 9-16) are shown. Blots were first probed with a DNA containing the hCHL2 ORF (CHL2), and then re-probed with human ß-actin (Clontech). Essentially identical results were achieved with three different blot batches. Lane 1, spleen; lane 2, thymus; lane 3, prostate; lane 4, testis; lane 5, uterus (without endometrium); lane 6, small intestine; lane 7, colon; lane 8, peripheral blood leukocytes; lane 9, heart; lane 10, whole brain; lane 11, placenta; lane 12, lung; lane 13, liver; lane 14, skeletal muscle; lane 15, kidney; lane 16, pancreas. (B) In situ hybridization for CHL2 in adult mouse tissues, shown in paired bright-field (left) and dark-field (right) panels. (a) Uterus (transverse section) showing CHL2 expression primarily in the myometrium. (b) Colon, showing weak CHL2 expression in serosal cells – a different pattern to that of CHL1 (Nakayama et al., 2001). muc, submucosa; mus, muscularis; end, endometrium; myo, myometrium.