Fig. 8. AlexaFluor594-conjugated Concanavalin A does not affect mesodermal cell proliferation or mesothelial cell migration, and is inherited by daughter cells. Brightfield (A,C-E) and fluorescent (B,F) photomicrographs of AlexaFluor594-ConA-labeled yolk sac mesoderm and allantoic mesothelium. (A,B) ConA-labeled yolk sac mesodermal cells at 20 hours after culture. Arrowhead points to structures which give these cells a vacuolated appearance. (C) Control unlabeled yolk sac mesodermal cells, 20 hours after culture. Arrowhead points to `vacuolated' region, whereas the arrow points to a filopodial projection often seen on both labeled and unlabeled cultured yolk sac mesodermal cells. (D) Example of a very minor cell population found in cultures of both labeled and unlabeled yolk sac mesodermal cells, but which were never labeled, and are thus possibly cells of blood island provenance. (E,F) ConA-labeled headfold-stage allantoic explant at 8 hours of culture. Arrows point to labeled mesothelial outgrowth. In this and Figs 9, 10, 11, d: distal, p: proximal. Scale bar in F: 50 µm (A-D); 100 µm (E,F).