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Fig. 11. Transient expression analysis of F3 and F8 cis-regions fused with the GFP reporter gene in transgenic zebrafish. Lateral and dorsal views of transgenic fish embryos transiently harboring the F3pGFP (A-E) and F8pGFP (F,G) constructs. GFP activity with F3pGFP at 8.5 (A), 12 (B) and 32 (E) hpf. Bright (C) and dark (D) field observations of F3pGFP transgenic fish at 18 hpf. F8pGFP expression at 18 (F) and (G) 30 hpf. a, anterior; di, diencephalon; e, eye; mhb, mid-hindbrain boundary; ov, optic vesicle; tec, tectum; tel, telencephalon; p, posterior.