Fig. 5. Developmental changes in lacZ expression with the F3placZ construct. Lateral and frontal views of the F3placZ transgenic embryos at 8.5 (A,B), 9.5 (C), 10.5 (D), 12.5 (I,J) and 13.5 (M) dpc, and a dorsal view at 13.5 dpc (N), following ß-galactosidase staining. Transverse sections of transgenic embryos at 10.5 (E-H) and 12.5 (K,L) dpc. Sagittal sections of embryos at 10.5 (H) and 12.5 (L) dpc. Whole-mount in situ hybridization analysis of noggin mRNA indicates that mouse noggin expression is evident in the roof of the neural tube, including at the level of the diencephalon (O, arrowheads). Lateral view of the F3hsplacZ transgenic embryo at 10.5 dpc, following ß-galactosidase staining (P). cp, choroid plexus; d, diencephalon; he, cortical hem; lt, lamina terminalis; lv, lateral ventricle; m, mesencephalon; t, telencephalon.