Fig. 3. Early pupal origin of subsets of neuronal and non-neuronal cells in the adult pharyngeal sense organs, as shown by BrdU incorporation at 3 hours APF in MJ94 (G: 18 hours APF). The patterns were visualized by lacZ expression (blue) in the light microscope (A,B,F,G) or by mCD8-GFP expression (green) in the confocal microscope (C-E,H,I). The anti-BrdU product is shown in brown (A,B,F,G) or red (C-E,H,I). (A-E) In the lso, BrdU label is particularly obvious in the neurons of the distal sensilla 8 and 9. Additional BrdU take-up is visible in the neuron of the mononeuronal sensillum 6 (D) and in subsets of accessory cells (C-E: arrows). (F-I) In both vcso (F,H) and dcso (G,I), two accessory cells (arrowheads) but none of the neurons (large arrows) are BrdU positive. However, BrdU label is present in the neurons of the fishtrap bristles (H: small arrows). Asterisks in G,H indicate BrdU uptake in cells of the pharyngeal wall. (A,C,D,E) Sagittal sections; all others panels represent frontal views. Dots indicate midline. Distal is to the left (A-E) or to the bottom (F-I). Scale bars, 10 µm (F,G), 20 µm (A,B,H), 40 µm (C-E,I).