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Fig. 3. Loss of GLP-1/Notch signaling causes increased distal GLD-1 accumulation. (A-C) Distal end (left) of dissected hermaphrodite gonad arms stained with DAPI (blue), GLD-1 specific antibodies (red) and REC-8 antibodies (not shown). gld-2(q497) gld-1(q361); unc-32(e189) glp-1(q175) animals (C), which lack GLP-1/Notch signaling, have high distal GLD-1 accumulation levels. (D,E) gld-1 mRNA spatial accumulation is similar in gld-2(q497) gld-1(q361); unc-32(e189) (D) and gld-2(q497) gld-1(q361); unc-32(e189) glp-1(q175) (E). gld-1 sense probe shows little or no staining (not shown). Scale bar: 20 µm.