Fig. 6. NOS-3 is required for fbf-1 fbf-2 double mutant Glp phenotype. (A) fbf-1(ok91) fbf-2(q704) and fbf-1(ok91) fbf-2(q704) nos-3(oz231) animals one day past L4 were dissected and stained with REC-8 (proliferative) and HIM-3 (meiotic) antibodies (Hansen et al., 2004). The graph shows the average number of cell diameters along the length of the gonad arm that cells are proliferative (REC-8, green) or meiotic (HIM-3, red). The proliferative zones of 8/10 fbf-1(ok91) fbf-2(q704) nos-3(oz231) arms were smaller or of similar size to those of wild-type, while 2/10 were much larger (33 and 40 cell diameters). The phenotype is independent of germline sex as fog-3(q443); fbf-1(ok91) fbf-2(q704); unc-32(e189) and fog-3(q443); fbf-1(ok91) fbf-2(q704) nos-3(oz231); unc-32(e189) animals were similar to the unfeminized animals (data not shown). Error bars = 1 s.d. (B) Dissected gonad arm of fog-3(q443); fbf-1(ok91) fbf-2(q704) nos-3(oz231); unc-32(e189) young adult animal stained with DAPI (blue), REC-8 (green) and GLD-1 (red). Distal is to the left. Scale bar: 20 µm. (C) Dissected gonad arms of wild-type (top) and gld-2(q497) fog-3(q443); fbf-1(ok91) fbf-2(q704) nos-3(oz231); unc-32(e189) (bottom) stained with DAPI (blue) and GLD-1 (red). Only a portion of the distal arms are shown with distal to the left.