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Fig. 4. Analysis of basement membranes in Lama1-/- embryos. (A,C,E) Wild type; (B,D,F) Lama1-/-. Arrowheads indicate the embryonic basement membrane, and arrows indicate RM, when present. (A,B) Triple staining for laminin {alpha}1 (green), laminin {alpha}5 (red), and nuclei (blue) at E5.5. Both control and mutant embryos have a laminin {alpha}5-positive embryonic basement membrane, but the mutant lacks immunoreactivity of {alpha}1 and RM. (C,D) Double staining for laminin {alpha}5 (red) and nuclei (blue) at E6.5. Laminin {alpha}5 is present in the embryonic basement membrane in both embryos. (E,F) Double staining for collagen {alpha}1, {alpha}2(IV) (green) and nuclei (blue). Collagen IV is present in both the embryonic basement membrane and RM in the control, but only the embryonic basement membrane is present in the Lama1 mutant. Asterisk in F probably represents non-basement membrane associated collagen IV made by PE, clustered as in Fig. 3J. Note that the arrangement of nuclei in B and F suggest that cavitation occurred in Lama1 mutant embryos. Scale bar in F: 100 µm.