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Fig. 6. Analysis of basement membranes in Lama1-/-; Mr5 embryos. (A-D) Embryos were double stained for laminin {alpha}1 (green) and laminin {alpha}5 (red). Arrowheads indicate the embryonic basement membrane, and arrows indicate RM, when present. (A,B) At E6.5, the Lama1-/-; Mr5 embryo (B) has laminin {alpha}5 in the embryonic basement membrane but lacks laminin {alpha}1 and RM, when compared with the control (A). (C,D) At E7.5, Lama1-/-; Mr5 embryos appear to still be alive and to have an embryonic basement membrane containing laminin {alpha}5. (E,F) Embryos were triple stained for laminin ß1 (red), cytokeratin 8 (green) and nuclei (blue). The Lama1-/-; Mr5 embryo is much smaller than the control, but both contain embryonic ectoderm (EE), extra-embryonic ectoderm (ExE) and ectoplacental cone (EPC). Trophoblast blood sinuses (S) are present in the control but are lacking in the Lama1-/-; Mr5 embryo. Scale bars: in D, 100 µm for A-D; in F, 100 µm for E,F.