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Fig. 7. Analysis of Mr5 transgene expression on the Lama5-/- genetic background. Sections were double stained for laminin {alpha}1 (green) and {alpha}5 (red). The red channel is shown in A and C, and both red and green channels are shown in B and D. (A,B) At E7.5, transgene-derived laminin {alpha}5 was detected in the embryonic basement membrane and in RM (A,B), but no {alpha}5 was detected in a Lama5-/- embryo that lacked the transgene (C,D). Scale bar: 100 µm.