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Fig. 1. Anti-phospho-SMAD1/5/8 immunofluorescence (red) of paraffin sectioned mid-dorsal skin at P5 (early anagen; A,B), P13 (full anagen; C,D), P16 (anagen-catagen transition; E), P18 (catagen; F), P20 (telogen; G) and P28 (first postnatal anagen; H). Nuclei are counterstained with Hoechst 33258 and appear blue. Presence of nuclear phospho-SMAD is indicated by yellow arrows. (B,D) Higher magnification photographs of the follicles shown in A and C, respectively. Diffuse red staining in the center of some follicles is due to autofluorescence of the hair shaft. ORS, outer root sheath; IRS, inner root sheath; DP, dermal papilla; SG, sebaceous gland.