Fig. 6. Effects of epidermal-specific deletion of Bmpr1a on expression of
regulators of hair shaft and IRS differentiation. (A-F) In situ hybridization
of mid-dorsal skin from K14-Cre40;
Bmpr1acl/+ control littermate (A,C,E) and
K14-Cre40; Bmpr1acl/cl mutant (B,D,F) at P8,
using the digoxigenin-labeled antisense probes indicated. Hybridization
signals (purple-brown) are indicated by arrows. Dark brown cells (A,E,F) are
pigmented. (G,H) Immunofluorescence of P12 dorsal K14-Cre40;
Bmpr1acl/+ control littermate (G) and
K14-Cre40; Bmpr1acl/cl mutant (H) skin with
anti-GATA3. (I,J) In situ hybridization of ventral head skin from
K14-Cre40; Bmpr1acl/+ control
littermate (I) and K14-Cre40; Bmpr1acl/cl mutant
(J) at P8 using 35S-labeled antisense probe for Msx1.
(K,L) In situ hybridization of mid-ventral P8 skin from bcre-32;
Bmpr1acl/+ control littermate (K) and
bcre-32; Bmpr1acl/null mutant (L) using
35S-labeled antisense probe for Lef1. Bright red regions
(K; white arrows) are due to reflection of light by the hair shaft. (M,N)
Immunofluorescence for ß-catenin (red) in P12 dorsal skin from
K14-Cre40; Bmpr1acl/+ control
littermate (M) and K14-Cre40; Bmpr1acl/cl mutant
(N). Arrows (A,C,E,F,I-L) indicate hybridization signals. Arrows (G,M,N)
indicate nuclear localized GATA3 and ß-catenin, respectively. Nuclei are
counterstained with Hoechst 33258 (I-N). Scale bar in N applies to A-N. DP,
dermal papilla.