Fig. 2. Histone H3 lysine 9 (H3/K9) methylation patterns in two-cell mouse embryos that were produced by fertilization, parthenogenesis and androgenesis. Mouse two-cell embryos that were collected 32 hours after in vitro fertilization (A), and parthenogenetic and androgenetic two-cell embryos (B), were subjected to immunocytochemistry with the antibody to methylated H3/K9 (MeH3K9). The antibody was localized with FITC-conjugated secondary antibodies (green), and DNA was stained with DAPI (blue). The parthenogenetic embryos were prepared by exposing MII-stage oocytes to 7% ethanol for 6 minutes, and collected for immunocytochemistry 32 hours later. The androgenetic embryos were prepared by fertilizing enucleated oocytes in vitro, and collected for immunocytochemistry 32 hours after fertilization. The experiments were conducted four times and similar results were obtained. More than 25 images were obtained for each type of embryo and representative examples are shown.