Fig. 4. Xbh1 enhances the RGC-inducing ability of atonal-related genes. (A,B) Retinas lipofected with Xath3+GFP or Xath3+Xbh1+GFP. (C,D) Percentage of RGCs (C) or photoreceptors (D) in retinae lipofected with GFP+pCS2 vector, or with GFP in combination with Xbh1, Xath5, Xath5+Xbh1, Xath3, or Xath3+Xbh1. The percent representation of each cell type was calculated as the average±s.e.m. Counted cells were: n=2625 cells from six retinae for GFP; n=2908 cells from six retinae for Xbh1; n=1087 cells from 10 retinae for Xath5; n=1375 cells from 11 retinae for Xath5+Xbh1; n=2018 from seven retinae for Xath3; and n=1406 from eight retinae for Xath3+Xbh1. Significant differences have been determined by one-way analysis of variance (ANOVA) with the Tukey-Kramer Multiple Comparisons Test as a post-test. Black asterisks represent significant differences (^**P<0.01, ^***P<0.001).