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Fig. 6. (A-D) Co-lipofection of Xath5 with Xbh1 and Xbh1Vp16 constructs. Xath5 was lipofected either with GFP+pCS2 (A), or with GFP+Xbh1 (B) or GFP+Xbh1Vp16 (C), and their effect analyzed on cell-type frequencies in transfected clones (D). Counted cells were: n=2625 cells from six retinae for GFP; n=2908 cells from six retinae for Xbh1; n=3707 cells from 19 retinae for Xbh1Vp16; n=885 from eight retina for Xath5; n=1368 cells from eight retinae for Xath5+Xbh1; n=1081 from five retinae for Xath5+Xbh1Vp16. Error bars represent s.e.m.; significant differences have been calculated by one-way analysis of variance with the Tukey-Kramer Multiple Comparisons Test as a post test (***P<0.001). Symbols are as in Fig. 3. (E) RT-PCR analysis of animal caps injected with different combinations of mRNA, as indicated. Caps injected with either Xath5 (500 pg) or Xbh1 (500 pg) show activation of Xbrn3d. By contrast, animal caps injected with Xath5 (500 pg)+Xbh1Vp16 (500 pg), or with Xbh1Vp16 (500 pg) alone show no activation of Xbrn3d expression.