Fig. 1. Mutants with reduced dMyc have a larval growth defect. (A) dm gene. The dm⁴ deletion, the positions of the P-elements used in the excision screen (in red), and the P-element and gypsy element that define the hypomorphic alleles dm^P1/P0 and dm¹, respectively, are indicated. Black boxes, coding region; arrow, the transcription start site. (B) Comparison of age-matched dm⁴/Y hemizygous mutants with control dm^1-14-2/Y (see text) hemizygous males. (C) RT-PCR analysis of RNA from larvae of the indicated genotypes, harvested at 24 hours AED, using primers to detect regions across the exon 2/3 boundary and within exon 3 of dMyc. CaMKII is a loading control. (D) Western blot of extracts from larvae of the indicated genotypes, harvested 24 hours AED.