Fig. 4. dMyc promotes and dMnt blocks endoreplication. Flp/Gal4 was used to clonally express dMyc (A-C,G-K) or dMnt (D-F) with GFP (green). DNA is stained with DAPI (blue). (A-F) Heat shock-induced clones in fat body from third instar larvae; DNA and GFP images are merged in C and F. (G) Quantification of nuclear DAPI fluorescence. DAPI stained fat body nuclei from larvae of the indicated ages were analyzed by deconvolution microscopy and heat shock-independent GFP marked dMyc-expressing cells were compared with neighboring non-GFP control cells. (H-K) Fat body from a second instar larva containing a heat shock-independent dMyc expressing FLP/Gal4 clone [marked with GFP (I) and outlined in white] was stained with DAPI (H) and anti-fibrillarin (J). DAPI and anti-fibrillarin images are merged in K.