Fig. 5. DNA replication and Cyclin E protein levels oscillate in dMyc-expressing cells. (A-D) Control larvae (A,C) and larvae expressing dMyc driven by Adh-Gal4 (B,D) were fed BrdU for 5 hours and dissected at late second instar. Fat body was stained with anti-BrdU (A,B) and DNA was visualized with DAPI (C,D). Arrowheads indicate nuclei that did not label with BrdU. (E,F) Fat body from third instar control larvae (E,G) or larvae expressing dMyc driven by Adh-Gal4 (F,H) was stained with anti-Cyclin E (E,F) and DAPI (G,H). Cyclin E is expressed at high (arrows) and low (arrowheads) levels in control and dMyc-expressing cells.